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Laser & Photonics reviews: super resolution microscopy based on spatial spectrum control

wallpapers Cruise 2020-08-26

optical microscope is non-invasive convenient. However the traditional microscope is equivalent to a low-pass filter its spectrum receiving range is limited by the objective's numerical aperture so the resolution is limited which can only reach about 200 nm horizontally. Super resolution microscopy can break through the resolution limit of traditional microscope has a wide range of applications in biology chemistry materials other fields.

Professor Liu Xu Professor Yang Qing of School of Optoelectronic Science engineering of Zhejiang University pioneered the frequency shift super-resolution microscopic imaging method. In 2017 the luminescent nanowires were used as the local light source skillfully utilized its strong light localization ability strong evanescent field coupled with the thin film waveguide with low propagation loss high refractive index realizing the large field of view high refractive index on-chip for the first time The resolution of the far-field unmarked super-resolution microscopy is 5 times higher than that of the traditional microscopy its field of view is 2 orders of magnitude larger than that of the previously reported unmarked far-field super-resolution microscopy. The paper was published in Physical Review letter

. Then in 2019 the research group improved on the original basis using organic light-emitting film instead of nanowire as evanescent field light source for on-chip super-resolution microscopic imaging. Organic light-emitting thin film has a wide spectrum of visible light emission characteristics combined with filter acquisition can achieve a wide range of frequency shift. At the same time the preparation process of organic light-emitting film is simple mature which has the advantage of large-scale processing. Combined with the spectral synthesis algorithm the team realized the resolution of 140 nm the reconstruction of complex samples for the first time. The paper was published in advanced functional material (DOI: 10.1002 / ADFM. 201900126).

recently the research group of Professor Liu Xu Professor Yang Qing Professor Ma Yaoguang reviewed the super-resolution imaging technology from a new perspective that is spatial spectrum modulation using high frequency evanescent field. The spatial spectrum modulation method is to transfer the high frequency information of the object to the spectrum receiving range of the traditional microscope objective by various means. The implementation methods include using special relay objective lens (super lens microsphere lens) to compress the high-frequency evanescent field information of the object to low frequency so that it can be received by ordinary objective lens; or using high-frequency evanescent field illumination the high-frequency part of the object space spectrum can be transferred to low frequency by frequency shift then spliced in the spectrum domain through the spectrum synthesis reconstruction algorithm to obtain the image In order to achieve higher resolution a wider spectrum coverage is obtained.

are compared with the point scan acquisition super-resolution imaging method which uses high-frequency evanescent field for spatial spectrum modulation uses wide field imaging method to acquire images so the imaging speed can be faster. In addition the imaging method is simple the super-resolution imaging can be realized by integrating a super lens or a frequency shift illumination chip on the traditional microscope. Super resolution chips based on frequency shift can be fabricated by IC compatible process with low cost in the future which has a wide application prospect.

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